Composite

Part:BBa_K4613307:Design

Designed by: Qinfei Zhou   Group: iGEM23_NAU-CHINA   (2023-10-10)


Plac-RBS-yf1-RBS-fixJ-Pfixk2-RBS-GFP(YF1-GFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 589
    Illegal NgoMIV site found at 661
    Illegal NgoMIV site found at 751
    Illegal NgoMIV site found at 769
    Illegal NgoMIV site found at 1281
    Illegal NgoMIV site found at 1574
    Illegal NgoMIV site found at 1668
    Illegal AgeI site found at 303
    Illegal AgeI site found at 1449
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1338
    Illegal BsaI.rc site found at 202
    Illegal BsaI.rc site found at 2808


Design Notes

Because of the delay of gene systhesis by GENERAL BIOL company, we didn't conduct any wet lab experiments about PAL and PH207.


Source

NAU-CHINA 2023

References